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Concentrating on Chromatography: Daily Summaries Delivered
by David Oliva
Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!
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- Today39 min
Episode 81: PFAS, 6PPD-Quinone & the Science of Emerging Contaminants w/ Arielle Cocozza (ALS USA)
Arielle Cocozza, National Technical PFAS Lead at ALS USA Environmental, joins David Oliva and Candi Gokey to break down the current state of PFAS analysis — from her unconventional path out of a physics degree into environmental chemistry, to standardizing PFAS methods across a national lab network.In this episode, Arielle explains what PFAS actually are and why their fluorine-carbon bonds make them so persistent, walks through LC-MS/MS vs. GC-MS/MS selection for different PFAS classes (including fluorotelomer alcohols and volatile fluorocarbons), and discusses column chemistry choices like C18 and pentafluorophenyl phases. The conversation dives deep into 6PPD-quinone — the tire-wear-particle degradation product linked to coho salmon die-offs — and how EPA fast-tracked Draft Method 1634 in response. Arielle also covers the Total Oxidizable Precursor (TOP) assay, EPA's new ultra-short direct-injection Method 1633/563 workflows, sample volume miniaturization, automation trends in extraction, and the patchwork of state-by-state PFAS regulations on food packaging and biosolids ahead of federal rulemaking.
- 1 weeks ago1h 0m
Episode 80: Untargeted Metabolomics & Ocean Chemistry with Mauricio Caraballo
Mauricio Caraballo, PhD, director of the MSCollaboratory at UC San Diego, joins Candi Gokey and host David Oliva to unpack how untargeted LC-MS/MS metabolomics reveals hidden chemistry in coral reefs, fish, and ocean water. They dig into molecular networking, GNPS, and how free mass spec training is helping marine scientists worldwide analyze samples they couldn't otherwise decode.Links: mscollaboratory.ucsd.edu | Mauricio Caraballo: linkedin.com/in/mauricio-caraballo-4b5577176
- 2 weeks ago30 min
Episode 79: Mechanical engineer Mason Chilmonczyk on fixing mass spec's bubbles, clogs, and leaks
Mechanical engineer Mason Chilmonczyk, PhD (Andson Biotech) joins host David Oliva to explain why sample prep — not the mass spec itself — is the biggest bottleneck in LC-MS and native MS workflows. Mason breaks down the DynaChip, a microfluidic device that shrinks hours of buffer exchange into seconds, and shares how "democratizing" mass spec is opening the door for cell therapy manufacturers to catch analytical blind spots that traditional workflows miss. A must-listen for anyone working in native mass spec, biopharma analytics, or cell therapy development.
- 3 weeks ago33 min
Episode 78: SPME, PFAS & Green Chemistry with Dr. Emanuela Gionfriddo
Dr. Emanuela Gionfriddo, Associate Professor of Chemistry at the University at Buffalo, joins host David Oliva to explore solid phase microextraction (SPME), green analytical chemistry, and PFAS analysis. Trained in Professor Janusz Pawliszyn's lab at the University of Waterloo — where SPME was invented — she shares how deep SPME theory reshaped her research and now drives her lab's work on environmental and biological chemistry.In this episode, Emanuela discusses how SPME complies with the 12 principles of green analytical chemistry, why her lab consistently produces the least waste in her department, and how biomimetic SPME devices using natural biopolymers like alginic acid and chitin reveal how pollutants bioaccumulate in the environment. She also covers her lab's PFAS research, including a surprising study testing mascara for PFAS contamination, the analytical challenges of volatile PFAS detection, and why field-deployable PFAS quantification isn't quite ready yet.Topics covered: SPME fundamentals, green analytical chemistry, PFAS detection, biomimetic extraction, mixed-mode sorbents, GC×GC for volatile PFAS, and emerging microplastics research.Connect with Dr. Emanuela Gionfriddo: https://www.linkedin.com/in/emanuela-gionfriddo-60492659/Subscribe to Concentrating on Chromatography for more conversations with leading separation science researchers.
- 4 weeks ago46 min
Episode 77: Hybrid Metabolomics Explained: Bridging Targeted & Untargeted MS with Bruker's Matthew Lewis
On this episode of Concentrating on Chromatography, hosts David Oliva @Organomation and Candice Gokey are joined by Matthew Lewis @Bruker to unpack the new hybrid metabolomics workflow born from Bruker's acquisition of @biocrates .For 20 years, mass spectrometrists have had to choose between targeted quantitation and untargeted discovery. Matthew explains how chemical derivatization, high-resolution mass spec, and Biocrates' 1,000+ metabolite kits now let researchers get both — without running samples twice.Topics covered: - Why "there's no such thing as an untargeted platform, just an uninformed one" - How the Biocrates MX Quant Kits standardize sample prep and derivatization - NMR vs. mass spectrometry for metabolomics — and why electrospray ionization is metabolomics' "biggest Achilles heel" - Data-dependent (DDA) vs. data-independent (DIA) acquisition, explained simply - Trapped Ion Mobility Spectrometry (TIMS) and collisional cross-section (CCS) as a "gas-phase chromatography" tool for resolving isomers and interferences - In silico derivatization software for annotating derivatized metabolites - The "digital metabolome archive": building future-proof, reanalysis-ready 4D data (MS1 + retention time + CCS + MS/MS) for AI-ready datasets - Why standardization — not just new features — is what will move the metabolomics field forwardMatthew also shares insights from Bruker's collaboration with Dr. Michael Witting at the Helmholtz Institute, and discusses the real-world sample constraints (biobanks, field research, precious cohorts) driving demand for do-more-with-less workflows.Guest: Matthew Lewis, Bruker — https://www.linkedin.com/in/matthew-reese-lewis/Co-host: Candice Gokey — https://www.linkedin.com/in/cmgokey15/Host: David Oliva, Organomation#Metabolomics #MassSpectrometry #Chromatography #Bruker #Biocrates #Lipidomics #SystemsBiology #TIMS #IonMobility
- Aug 31, 202642 min
Episode 76: PFAS Are Everywhere — And 99% Go Undetected | Dr. Viktoria Müller
If you take a handful of dirt from the roadside, it contains PFAS. The challenge isn't finding forever chemicals — it's finding all of them. Standard monitoring methods cover 40 to 50 PFAS species. Thousands more go completely unmeasured. That's the gap Dr. Viktoria Müller has spent her career trying to close.Viktoria is a postdoctoral researcher at the University of Graz specializing in PFAS environmental fate, transport, and the fluorine mass balance. Her work spans ski slopes, rivers, wastewater treatment plants, bees, wild deer, shrimp, and the Ganges River — and it has made international headlines. In this episode, she breaks down what we're missing, why routine analysis creates a false sense of security, and what wildlife can tell us that instruments alone cannot.In this episode:- [00:01:30] Why PFAS is unavoidable — and what you can actually control- [00:02:00] The pizza box explanation: how Viktoria describes PFAS to non-scientists- [00:04:00] How her research path built from soil → ski slopes → snowmelt → rivers → wildlife- [00:05:00] What "closing the fluorine mass balance gap" actually means for environmental monitoring- [00:06:50] Ski wax and the 99% problem: targeted PFAS analysis only identified less than 1% of the total organofluorine in ski wax — what is the rest?- [00:11:00] LC-MS/MS vs. ICP-MS/MS vs. CIC for PFAS and fluorine analysis — and which one Viktoria would buy ("don't blame me later")- [00:18:50] TOP assay vs. ozonation: same precursor, different breakdown products — what that means for anyone relying on TOP assay data- [00:21:00] Honeybees as short-term local PFAS sentinels — and how a beekeeper biologist changed how Viktoria interpreted her own data- [00:24:00] Collectors or house cleaners? How to tell whether an animal is accumulating PFAS or just carrying it on the surface- [00:25:30] The roe deer liver study: 87% drop in PFAS from 1998 to 2022 — bans work, but the industry keeps replacing regulated compounds with unregulated ones- [00:33:00] Wastewater treatment plants downstream: more PFAS species coming out than going in — and what the mass balance approach revealed about why- [00:37:00] Personal aside: Viktoria's one regret about not taking her own blood sample before she started working with PFAS- [00:39:30] What's next: a funded proposal exploring PFAS in clouds and atmospheric chemistry — a topic almost no one is working onConnect with Dr. Viktoria Müller:Google Scholar: https://scholar.google.com/citations?user=Prq41XUAAAAJ&hl=enLinkedIn: https://www.linkedin.com/in/viktoria-m%C3%BCller-8a1843a7/About Organomation:Organomation manufactures nitrogen blowdown evaporators and nitrogen generators used in PFAS sample preparation workflows, including EPA Methods 533, 537.1, and 1633. Learn more at organomation.com. @ChromatographyTalk is proudly produced by @Organomation #PFAS #ForeverChemicals #AnalyticalChemistry #EnvironmentalChemistry #MassSpectrometry #Chromatography
- Aug 24, 202642 min
Episode 75: Breathomics, Asthma & GC×GC: PH Stefanuto on Fighting the “Proof‑of‑Concept” Trap
In this episode of Concentrating on Chromatography, David Oliva and co‑host Candi Gokey sit down with Dr. Pierre‑Hugues (PH) Stefanuto to explore how comprehensive GC×GC‑TOFMS breathomics is reshaping asthma research and personalized medicine. PH shares his journey from forensic applications (yes, even the “smell of death”) to non‑invasive exhaled breath analysis for chronic lung inflammation, and why he believes asthma is the perfect playground to translate breath research into real clinical impact.We dig into PH’s recent work on a complete GC×GC‑TOFMS workflow to improve asthma monitoring, including patient breath collection, VOC analysis, biomarker discovery, and robust data processing strategies. He explains how combining targeted markers like FeNO with VOC “breathprints” can improve asthma phenotyping and treatment decisions, and how his group identified potential VOC biomarkers such as hexane, 1‑propanol, 2‑hexanone, undecane, and nonanal.A major theme of this conversation is PH’s call to “fight the breath proof‑of‑concept paradigm.” Instead of stopping at small, under‑powered studies, his group pushes through large cohorts, rigorous validation, and clinically relevant study design so that breath analysis can actually make it into the clinic and, eventually, into patients’ homes. We also talk candidly about skepticism toward breath analysis, how overpromising has hurt the field, and what it takes to rebuild trust with clinicians and medical staff.We also cover:Why the Multidimensional Chromatography Workshop (MDCW) was designed to be a free, open‑access event, and how that philosophy supports open science, networking, and inclusion of early‑career scientists and lab staff.PH’s path from forensic GC×GC to clinical breathomics and chronic inflammation research, and how CSI‑inspired curiosity turned into a career in analytical chemistry.How untargeted molecular screening and rich VOC fingerprints complement conventional single‑marker tests and enable finer‑grained patient phenotyping.Why GC×GC‑MS often achieves higher annotation rates than LC‑MS in certain metabolomics applications, thanks to retention patterns, EI libraries (NIST/Wiley), and structured 2D chromatography.The future vision: patients blowing into a simple device (or even a phone‑connected system) to monitor lung inflammation at home, integrate with wearables and environmental data, and guide day‑to‑day asthma management.How AI and machine learning can help connect small‑molecule breath data to larger metabolic pathways and multi‑omics datasets, inspired in part by platforms like GNPS.PH’s thoughts on chromatography education, why analytical chemistry is underrepresented in curricula, and how we should prepare the next generation of clinicians and scientists for omics‑driven healthcare.If you’re interested in chromatography, mass spectrometry, breath analysis, asthma, or translational metabolomics, this is a deep yet accessible conversation that bridges instrumentation, statistics, and real‑world clinical needs.Keywords: breathomics, exhaled breath analysis, asthma monitoring, GCxGC, GC×GC‑TOFMS, multidimensional chromatography, volatile organic compounds, VOC biomarkers, untargeted metabolomics, personalized medicine, PH Stefanuto, Pierre‑Hugues Stefanuto, MDCW, Multidimensional Chromatography Workshop, FeNO, chronic lung inflammation, clinical mass spectrometry, analytical chemistry education, chromatography podcast, Concentrating on Chromatography, David Oliva, Candi Gokey
- Aug 17, 202645 min
Episode 74: Decoding the Glycocode: AI-Powered Glycoproteomics & Cancer Research | Jacob Russell (Riley Lab, UW)
In this episode of Concentrating on Chromatography, host David Oliva is joined for the first time by co-host Candice (Candi) Gokey — PhD candidate at UC San Diego / San Diego State University and expert in LC-MS/MS method development for untargeted metabolomics to interview Jacob Russell, a third-year PhD student in the Riley Research Group at the University of Washington (Seattle).Jacob's research sits at the frontier of mass spectrometry based intact glycoproteomics, where the goal is to keep glycans attached to peptides during analysis — preserving the biological information that is lost when glycans are enzymatically removed. His lab, led by Prof. Nick Riley, develops new methods to tackle every stage of glycoproteomic analysis, from sample preparation and enrichment through data acquisition and bioinformatics.🔬 WHAT WE COVER:• What is the glycocalyx, and why does glycoproteomics matter for cancer, immunity, and protein biology?• N-linked vs. O-linked glycosylation: why they require completely different fragmentation strategies (HCD vs. ETD/EThcD)• Autonomous Dissociation-type Selection (ADS): how real-time library searching (RTLS) of oxonium ion ratios (m/z 138 vs. 144) enables on-the-fly selection of the right fragmentation method for N- vs. O-glycopeptides — published in J. Proteome Research (Sutherland, Veth, Russell et al., 2024)• How XGBoost machine learning outperforms RTLS by classifying ~50 oxonium ions simultaneously, recovering Core 2 O-glycopeptides that the simpler ratio-based method misses• Casanovo Foundation: a transformer-based foundation model for tandem mass spectra and how deep learning is reshaping glycopeptide classification (Sanders et al., arXiv 2025)• LacNAc-ase enabled glycoproteomics: using endo-β-galactosidase (EBG) to "trim" poly-LacNAc chains on N-glycans — uncovering previously undetectable glycoforms (ASMS 2026 presentation)• Cutaneous vs. uveal melanoma: comparing poly-LacNAcylated glycoproteins (including galectin-3 binding partners like CD63, LAMP-2, and basigin) between cell lines — and why uveal melanoma has a ~50% distant metastasis rate vs. ~5% for cutaneous • The future of intelligent data acquisition and real-time mass spectrometry• Candi's parallel world of untargeted metabolomics, coral-algae chemical communication, and GNPS/MassQL — and the surprising overlap with glycoproteomics🔗 RESOURCES MENTIONED:• Riley Research Group at UW: https://www.riley-research.com/• ADS Paper: Sutherland, Veth, Russell et al., J. Proteome Res. 2024, 23, 5606–5614• Casanovo Foundation: Sanders et al., arXiv 2025• GlyCounter software (Riley Lab, UW)• XGBoost (Chen & Guestrin, UW)• GNPS / MassQL (Dorrestein Lab, UCSD)👥 GUESTS:Jacob H. Russell — PhD Student, Dept. of Chemistry, University of Washington | Riley Research Group | Glycoproteomics | Mass Spectrometry | Machine LearningCo-host: Candice Gokey — PhD Candidate, UC San Diego / San Diego State University | LC-MS/MS Method Development | Untargeted Metabolomics | Coral Reef Holobiont Research🎙️ HOST: David Oliva | @ChromatographyTalk | @Organomation | @SeparationScience collaborator━━━━━━━━━━━━━━━━━━━━━━━━━━━━━🔔 Subscribe for more interviews with researchers and industry leaders in analytical chemistry, chromatography, and mass spectrometry.#Glycoproteomics #MassSpectrometry #Chromatography #AnalyticalChemistry #MachineLearning #Glycans #Proteomics #Cancer #UVealMelanoma #LCMS #OxoniumIons #ETD #XGBoost #RileyLab #UniversityOfWashington #PodcastScience #Metabolomics```
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About Concentrating on Chromatography
Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!
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